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本文采用军事医学科学院生产的多功能系统，对叮咬经不同抗原免疫接种兔后的中华硬蜱中肠消化细胞进行形态学测量分析，以了解蜱中肠受损程度和各种抗原诱导免疫力产生的强弱。结果表明：蜱血餐后其中肠增粗，肠壁增厚：消化细胞数量增加，体积增大；而细胞核相对较小，核体密度、表面积密度变小。中华硬蜱叮咬免疫兔后上述各种吸血后的正常反应不如佐剂对照组，尤以纯化抗原接种组明显，其中肠也增粗，由于大量消化细胞受损伤而脱落，使得消化细胞数减少，由于大量消化细胞受损脱落使消化细胞数减小和消化细胞平均截面面积减小。由于核固缩、核碎裂、核溶解早于细胞碎裂脱落，使细胞核的体密度和表面积密度明显减小。与对照组相比，肠直径、肠壁面积、消化细胞截面数、数面密度、截面面积和消化细胞核的体密度及表面积密度均有显著性或非常显著性差异。受损程度依次为纯化抗原（ＰＡ）组、中肠抗原（ＭＡ）组、唾液腺抗原（ＳＧＡ）组和全虫抗原（ＷＴＡ）组。卵抗原（ＯＡ）组损伤最轻。本实验的五个免疫接种组中有四个组能有效诱导宿主产生免疫力，以１０５ＫＤ纯化抗原接种作用最强，在选择制备抗蜱疫苗时可供参考
In this paper, the multi-functional system produced by the Academy of Military Medical Sciences was used to measure the morphological changes of midgut digestive cells of Ixodes sinensis after bite rabbits vaccinated with different antigens in order to understand the degree of damage in the midgut of ticks and the induction of immunity by various antigens The strength of The results showed that after the meal blood meal, the intestine was thick and the intestinal wall was thickened: the number of digestive cells increased and the volume increased; while the nucleus was relatively small, the density of nucleus and surface became smaller. Chinese Ixodes bite immune rabbits after the various blood-sucking normal reaction than the adjuvant control group, especially in the purified antigen inoculation group was obvious, which also thickening of the intestine, due to a large number of digestive cells damaged and shedding, making the digestion of cells reduced, As a large number of digestive cells damaged off the number of digestive cells and digested cells reduced the average cross-sectional area. As the nuclear pyknosis, nuclear fragmentation, nuclear dissolution earlier than the cell fragmentation shedding, so that the nuclear density of the body and surface area density was significantly reduced. Compared with the control group, there were significant or very significant differences in intestinal diameter, intestinal wall area, number of digested cell sections, number area density, cross-sectional area and body density and surface area density of digested nuclei. The order of damage was purified antigen (PA) group, midgut antigen (MA) group, salivary gland antigen (SGA) group and all insect antigen (WTA) group. Egg antigen (OA) group, the lightest damage. Four of the five immunized groups in this experiment can effectively induce the host to produce immunity, the strongest effect is to inoculate the 105KD purified antigen, and the reference for preparing the anti-tick vaccine