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目的:探讨甲基莲心碱对紫外线致人成纤维细胞形态结构的影响,为防治皮肤光老化性疾病提供实验依据。方法:建立中长波紫外线辐射人皮肤成纤维细胞光老化模型,实验分为对照组、模型组(单纯紫外线照射)和药物组(紫外线照射加甲基莲心碱干预),观察和分析成纤维细胞经紫外线照射后细胞生长形态和超微结构变化。结果:紫外线照射成纤维细胞后,细胞失去正常形态,超微结构显示线粒体损伤,而经甲基莲心碱处理后细胞损伤明显减轻。结论:适当浓度(0.8μM)的甲基莲心碱能减轻紫外线照射后成纤维细胞的结构损伤,为防治皮肤光老化疾病提供了新的治疗思路。
Objective: To investigate the effect of neferine on the morphology and structure of human fibroblasts induced by ultraviolet light and to provide experimental evidence for the prevention and treatment of skin photoaging diseases. Methods: To establish a photo-aging model of human skin fibroblasts with medium and longwave ultraviolet radiation. The experiment was divided into control group, model group (ultraviolet light only) and drug group (ultraviolet irradiation and neferine intervention) Cell growth morphology and ultrastructure changes after UV irradiation. RESULTS: After UV irradiation, fibroblasts lost their normal morphology and showed ultrastructural damage of mitochondria. However, the cell injury was significantly reduced after treatment with neferine. Conclusion: Neferine at a proper concentration (0.8μM) can reduce the structural damage of fibroblasts after UV irradiation and provide a new therapeutic approach for the prevention and treatment of skin photoaging diseases.