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目的:研究应用超顺磁氧化铁(SPIO)标记骨髓基质干细胞(MSCs)移植治疗帕金森病(PD)大鼠后的在体MRI观察。方法:分离、获取大鼠骨髓基质细胞,脂质体转染法将SPIO标记MSCs;制作PD模型,SPIO标记的MSCs移植到PD大鼠右侧纹状体区,应用MRI在体观察脑内移植的骨髓基质细胞的存活和迁徙情况。结果:体外SPIO标记的骨髓基质细胞普鲁蓝染色阳性;脑内移植SPIO标记MSCs的PD大鼠磁共振T2和T2GRE扫描检查显示在移植区呈低信号改变。随时间的延长,移植区信号向周围扩大。脑纹状体区的铁染色也可见SPIO标记MSCs从移植部位向四周迁移。结论:SPIO可用于体外标记MSCs,通过MRI技术可以对标记细胞脑内移植后进行初步的活体示踪,有利于MSCs移植治疗PD后的疗效观察。
OBJECTIVE: To study the in vivo MRI of Parkinson’s disease (PD) rats after transplantation of SPIO-labeled bone marrow stromal stem cells (MSCs). METHODS: Rat bone marrow stromal cells were isolated and transfected into SPIO-labeled MSCs by lipofection method. PD model and SPIO-labeled MSCs were transplanted to the right striatum of PD rats. MRI was used to observe intracerebral transplantation Of bone marrow stromal cells survival and migration. Results: SPIO-labeled bone marrow stromal cells were positive for Prussian blue staining. T2 and T2GRE scan of PD rats with SPIO-labeled MSCs transplanted in the brain showed a low signal change in the transplantation area. As time goes by, the signal in the transplant area expands to the periphery. Iron staining in the striatum of the striatum also demonstrated the migration of SPIO-labeled MSCs from the transplanted site to the periphery. CONCLUSIONS: SPIO can be used to label MSCs in vitro. Preliminary MRI tracing of labeled cells can be performed after transplanted into brain. It is helpful for the observation of the therapeutic effect of MSCs transplantation after PD.