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本文报导用半微量区别染色细胞毒性试验(微量-DiSC)检测血癌的体外化学药物敏感性,认为该试验可预测细胞毒药物对血癌的疗效。方法:首先抽取血癌患者的周围血液5—10ml 和骨髓(0.2—1.0μl),分离白细胞,放入含有各种药物浓度的磷酸缓冲液(PBS)培养液中,在含有5%CO_2的空气、一个大气压、37℃下孵育4天,随后把这种细胞和含有已知一定数量的鸭红细胞坚牢绿-苯胺黑染色液1:1混合,在室温下放置10分钟后,
This article reports the in vitro chemosensitivity of blood cancer using a semi-micro-differential staining cytotoxicity assay (micro-DsiSC) that predicts the efficacy of cytotoxic drugs on blood cancers. Methods: Peripheral blood 5-10ml and bone marrow (0.2-1.0μl) were collected from leukemia patients. Leukocytes were isolated and placed in phosphate buffered saline (PBS) medium containing various drug concentrations. The cells were cultured in air with 5% CO 2, After one-day incubation at 37 ° C for 4 days, the cells were mixed with 1: 1 stained green-aniline black stain containing a known amount of duck red blood cells and left at room temperature for 10 minutes.