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以块体SiO2大孔材料为基质,甲基丙烯酸缩水甘油酯(GMA)和甲基丙烯酸羟乙酯(HEMA)为功能单体,通过原位聚合和溶剂蒸发制备P(GMA-co-HEMA)/SiO2大孔复合材料,用SEM、EDS、BET、FTIR和TGDTA对样品进行表征,并将其用于固定褶皱假丝酵母脂肪酶(CRL)。结果表明:SiO2大孔材料很强的毛细管作用使共聚物均匀地涂敷在其孔壁上,形成P(GMA-co-HEMA)/SiO2复合纳米薄膜。共聚物的负载量和亲疏水性可分别通过改变单体浓度和体积比进行调控,当单体体积浓度为10%、GMA和HEMA的体积比为9∶1时大孔复合材料固定化脂肪酶比酶活达到最高,为3 886.9U/g,与底物反应重复操作8批次后剩余酶活率为68.7%。
P (GMA-co-HEMA) was prepared by in-situ polymerization and solvent evaporation using bulk SiO2 macroporous materials as matrix, glycidyl methacrylate (GMA) and hydroxyethylmethacrylate (HEMA) / SiO2 macroporous composites, the samples were characterized by SEM, EDS, BET, FTIR and TGDTA and used to immobilize Candida pleurotus lipase (CRL). The results show that the strong capillary action of SiO2 macroporous material makes the copolymer be uniformly coated on the pore walls to form P (GMA-co-HEMA) / SiO2 nanocomposite films. The loading of the copolymer and the hydrophilicity and hydrophobicity can be controlled by changing the monomer concentration and the volume ratio respectively. When the volume concentration of the monomer is 10% and the volume ratio of GMA to HEMA is 9:1, the immobilized lipase ratio Enzyme activity reached the highest, 3 886.9U / g, and the substrate reaction repeated operation 8 batches of the remaining activity was 68.7%.