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1 引 言 银杏是我国的特产,近年来国内外学者研究发现银杏种仁含有许多药用价值的成分.由于种胚中含有氢氰酸,食用过多容易中毒.本文采用在银杏采收后立即用低温速冻或60Co幅照抑制种胚生长发育,减少氢氰酸含量,并用酶水解样品后,直接用离子选择性电极法测定.结果显示RSD<2％,回收率达到89.5％～103％,方法简便,快速,测定结果较为满意.2 实验部分2.1 主要仪器和试剂 EA-940离子计,氰离子选择性电极,甘汞电极(美国orion公司),α-淀粉酶300U/mL,β-淀粉酶 280 U/mL(生化试剂),0.025 mol/L KH2PO4+0.025 mol/L Na2HPO4缓冲液(pH=6.9),10-1mol/L氰化钾标准贮备液,试剂均为分析纯.
1 Introduction Ginkgo biloba is a specialty of our country, in recent years, domestic and foreign scholars have found that Ginkgo biloba contains many medicinal value of ingredients.Because embryo contains hydrocyanic acid, too much consumption is easy to poison.This paper used in Ginkgo biloba Low temperature freezing or 60Co irradiation inhibited the growth of embryo, reduced the content of hydrocyanic acid, and hydrolyzed the sample with enzyme directly by ion selective electrode method.The results showed that RSD <2%, the recovery rate reached 89.5% ~ 103% Simple, rapid, satisfactory results.2 Experimental 2.1 Main instruments and reagents EA-940 ion meter, cyanide ion selective electrode, calomel electrode (orion company), α-amylase 300U / mL, β-amylase 280 U / mL (biochemical reagent), 0.025 mol / L KH2PO4 + 0.025 mol / L Na2HPO4 buffer (pH = 6.9) and 10-1 mol / L potassium cyanide standard stock solution.