以大豆品种合丰25的球形期体细胞胚为受体,以CpTI基因为目的基因,应用基因枪法进行了遗传转化,同时以抗性体细胞胚筛选率作为转化率的指标,对影响基因枪转化的几个参数进行了优化研究。结果表明,氯化钙浓度2.5 mol/L、氦气压力1 100 Psi、轰击次数为2次,有利于提高转化率;经卡那霉素筛选得到抗性植株,经PCR分析鉴定有2株为阳性,初步证明外源基因CpTI已转到大豆基因组内。 Using the somatic embryogenesis of soybean variety Hefeng25 as the receptor, the CpTI gene was used as the target gene and genetically transformed by the gene gun method. At the same time, the screening rate of somatic embryos was taken as the index of transformation rate, Several parameters of conversion were optimized. The results showed that calcium chloride concentration of 2.5 mol / L, helium pressure of 1 100 Psi, bombardment times of 2 times were conducive to improve the conversion rate; kanamycin-resistant plants were screened by PCR analysis identified two strains as Positive, preliminary evidence that the exogenous gene CpTI has been transferred to the soybean genome.