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利用双向电泳、ＳＤＳ一聚丙烯酰胺凝胶电泳分子量测定、钙结合蛋白染色等钙结合蛋白的检测技术揭示，烟草中功能未知的病程相关蛋白（Ｐａｔｈｏｇｅｎｅｓｉｓｒｅｌａｔｅｄｐｒｏｔｅｉｎｓ，ＰＲｓ）具有钙离子结合活性，其电泳迁移率、分子量测定等特性受到钙离子的显著影响；含量最高的未知功能蛋白ＰＲｂｌ，ＰＲｂ２在低钙和高钙条件下均表现钙结合活性，而ＰＲｂ７和ＰＲｂ８（两和几丁质酶）仅在低钙状态下表现一定的钙结合能力；并发现在抗病毒诱导剂（８８－Ｄ）作用下，细胞间隙提取液中出现其他钙结合物质。
Two-dimensional electrophoresis, SDS-polyacrylamide gel electrophoresis molecular weight determination, calcium binding protein staining and other calcium-binding protein detection technology revealed that Tobacco unknown function of the disease-related proteins (Pathogenesisrelatedproteins, PRs) with calcium ion binding activity, Rate and molecular weight were significantly affected by calcium ion. The highest content of unknown functional proteins PRb1 and PRb2 showed calcium-binding activity under low calcium and high calcium conditions, while PRb7 and PRb8 (two and chitinase) Calcium-binding ability was observed under low-calcium condition. Other calcium-binding substances appeared in the cell gap extract under the action of antiviral inducer (88-D).