用离子交换和凝胶过滤层析技术从长白山白眉蝮蛇蛇毒（Ａｇｋｉｓｔｒｏｄｏｎｂｌｏｍｈｏｆｆｉｕｓｕｒｅｎｓｉｓ）中分离得到了Ｌ氨基酸氧化酶（Ｌａｍｉｎｏａｃｉｄｏｘｉｄａｓｅ），经ＳＤＳ聚丙烯酰胺凝胶电泳（ＳＤＳＰＡＧＥ）和基体辅助激光解吸电离飞行时间质谱（ＭＡＬＤＩＴＯＦＭＳ）鉴定，发现Ｌ氨基酸氧化酶由两个不相等的亚基组成，一个亚基的分子量约为３６０００，另一个亚基分子量约为５７０００．以Ｌ亮氨酸作为底物进行Ｌ氨基酸氧化酶活力测定时发现，其反应的适宜ｐＨ值为４５５和８９，紫外吸收光谱明显表现出核黄素蛋白的特征 Lamino acid oxidase (Laminoacidoxidase) was isolated from Agkistrodon bomhoffiusurensis by ionexchange and gel filtration chromatography. SDS  polyacrylamide gel electrophoresis (SDSPAGE) Assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification and found L amino acid oxidase consists of two subunits are not equal, a molecular weight of about 36,000, another subunit molecular weight of about 57,000. L  leucine as a substrate for L  amino acid oxidase activity assay found that the appropriate pH value of the reaction was 45  5 and 89, the UV absorption spectrum clearly shows the characteristics of riboflavin protein