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采用分光光度法测定柯柯豆碱的含量，在272．5nm波长处有最大吸收峰，浓度在2．2～15．3μg／ml（r=0．9993）呈良好线性关系，RSD=0．98％（n=32）。重现性、精密度、稳定性良好。
The spectrophotometric method was used to determine the content of collocacin, which had the maximum absorption peak at 272.5 nm. The linear range was 2.2 ~ 15.3 μg / ml (r = 0.9993), RSD = 0. 98% (n = 32). Reproducibility, precision and stability are good.