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通过静电纺丝技术,调控溶剂种类和溶液浓度,制备了圆柱状无孔纤维、条带状多孔纤维和圆柱状多孔纤维。通过扫描电镜观察纤维形貌,通过物理吸附仪探究纤维比表面积。并将3种纤维膜进行固定化酶,探究其最适反应温度、最适反应pH、存储稳定性和重复使用稳定性。最终进行了染料降解性能测试。结果表明:多孔有利于提高纤维的比表面积。固定化酶和游离酶的最适反应温度为50℃,最适反应pH=3.5,固定化酶有利于提高酶的存储稳定性和重复使用稳定性。较游离酶和其他形貌纤维,圆柱状多孔纤维固定化酶后具有最好的染料降解性能。
Through the technology of electrospinning, the type of solvent and the solution concentration were controlled to prepare cylindrical non-porous fibers, ribbon-shaped porous fibers and cylindrical porous fibers. The morphology of the fibers was observed by scanning electron microscopy and the specific surface area of the fibers was explored by physical adsorption machine. The three kinds of fiber membranes were immobilized to enzyme, and the optimal reaction temperature, optimum reaction pH, storage stability and repeated use stability were explored. The final dye degradation performance test. The results show that: porous is conducive to increase the fiber surface area. The optimal reaction temperature of immobilized enzyme and free enzyme is 50 ℃, and the optimum reaction pH is 3.5. Immobilized enzyme is helpful to improve the storage stability and reusable stability of enzyme. Compared with free enzymes and other morphological fibers, cylindrical porous fibers have the best dye degradation properties.