可见及紫外分光光度法在微量元素的定量分析中、在不饱和有机化合物及芳香族化合物的定量分析中占有重要地位,得到了广泛应用。但遗憾的是:该法迄今仍未摆脱仪器分析中使用标准样品绘制校正曲线的传统做法。本文试图摆脱对标准样品的依赖,开辟一条无标样可见紫外分光光度法的新道路。将锐线光源运用于可见及紫外分光光度计,所测得的吸收系数 Visible and UV spectrophotometry in the quantitative analysis of trace elements, unsaturated organic compounds and aromatic compounds in the quantitative analysis plays an important role, has been widely used. Unfortunately, however, the law has so far not outgrown the traditional practice of using standard samples for the calibration curve in instrumental analysis. This article attempts to get rid of the reliance on the standard sample, open up a new way of standard UV spectrophotometry. The sharp line of light source used in visible and UV spectrophotometer, the measured absorption coefficient