检测 Mdrl基因表达水平可预测白血病化疗效果 ,用原位杂交的方法可检测 Mdrl在单个细胞的表达水平。本文用Rt- PCR的方法获得了一段特异的 c DNA片段 ,将其克隆到 PGEM4Z载体中 ,经 DNA序列分析证明与文献报道一致。采用地高辛素 (DIG) RNA标记试剂盒制备反义 RNA探针 ,已初步用于临床骨髓涂片标本的原位杂交检测 Detection of Mdrl gene expression level can predict the efficacy of leukemia chemotherapy, in situ hybridization method can detect Mdrl in a single cell expression levels. In this paper, a specific fragment of c DNA was obtained by Rt-PCR and cloned into PGEM4Z vector. The result of DNA sequence analysis showed that it was consistent with the reported in the literature. Preparation of antisense RNA probe with digoxin (DIG) RNA labeling kit has been initially used for in situ hybridization detection of clinical bone marrow smear specimens