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目的观察丁香叶提取物(folium syringae extract,FSE)对CCl_4诱导的药物性肝损伤的保护作用。方法在2014年1月—2016年1月研究将40只Wistar大鼠随机分为正常对照组、模型组、FSE低剂量组、FSE中剂量组、FSE高剂量组,模型组用CCl_4制备慢性肝损伤大鼠模型,FSE低剂量组、FSE中剂量组、FSE高剂量组预防性给药7 d,24 h后处死,分别测定血清中ALT、AST,血清和肝匀浆中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量,并相互比较。取肝组织病理切片进行病理学比较。计量资料比较采用t检验,P<0.05为差异有统计学意义。结果 FSE低、中、高剂量组均能够降低药物性肝损伤大鼠血清ALT、AST水平,与模型组比较差异均有统计学意义(均P<0.05)。血清中,FSE低、中、高剂量组均能提高药物性肝损伤大鼠血清中SOD含量,降低MDA含量,提高GSH-Px含量,其结果与模型组比较差异均有统计学意义(均P<0.05)。肝匀浆中,FSE高剂量组能提高SOD活性,降低MDA含量,提高GSH-Px含量,与模型组比较差异均有统计学意义(均P<0.05)。结论丁香叶提取物对CCl_4诱导的大鼠药物性肝损伤具有保护作用。
Objective To observe the protective effect of Folium syringae extract (FSE) on CCl_4-induced drug-induced liver injury. Methods From January 2014 to January 2016, 40 Wistar rats were randomly divided into normal control group, model group, low-dose FSE group, middle-dose FSE group and high-dose FSE group. Chronic liver The rats in the injury model, FSE low dose group, FSE middle dose group and FSE high dose group were given prophylactically for 7 days and then sacrificed 24 hours later. Serum ALT, AST, serum and liver homogenate superoxide dismutase superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were measured and compared with each other. Pathological sections of liver were taken for pathological comparison. Measurement data were compared using t test, P <0.05 for the difference was statistically significant. Results The levels of serum ALT and AST in serum of rats with drug-induced liver injury were significantly lower than those of model group (all P <0.05). Serum FSE low, medium and high dose groups can improve the serum SOD content, reduce the content of MDA and increase the content of GSH-Px in rats with drug-induced liver injury, the results were significantly different from the model group (P <0.05). Liver homogenate, high-dose FSE group can increase SOD activity, reduce MDA content, increase GSH-Px content, compared with the model group, the difference was statistically significant (P <0.05). Conclusion Clove leaf extract has a protective effect on CCl_4-induced drug-induced liver injury in rats.