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目的:探讨重组人酸性成纤维细胞生长因子对微波灼伤创面组织细胞的促增殖作用。方法:运用NS-FⅡ型多功能光谱治疗仪器建立微波灼伤动物模型,分为5组,每个创面按分组分别滴加0．9％氯化钠注射液(NS)、重组人表皮生长因子(rh-EGF,5 mg·L-1)、重组人酸性成纤维细胞生长因子(rh-aFGF,5mg·L-1)、重组人碱性成纤维细胞生长因子(rh-bFGF,5 mg·L-1)和牛脑提取液(bBE,1O mg·L-1)各0．2 ml,直到伤后第5d,取创面组织经流式细胞仪检测全层皮肤组织细胞周期及DNA含量。结果:应用生长因子各组的SPF、PI及DNA含量均明显高于NS组(P<0．05)。结论:重组人酸性成纤维细胞生长因子具有促进微波灼伤创面组织细胞增殖作用,促进创面的修复,缩短创面愈合时间。
Objective: To investigate the effect of recombinant human acidic fibroblast growth factor on the proliferation of wound tissue after microwave burns. Methods: The animal model of microwave burns was established by using NS-F Ⅱ multi-function spectrophotometer. The wounds were divided into 5 groups. Each wound was treated with 0.9% sodium chloride injection (NS), recombinant human epidermal growth factor (rh-EGF, 5 mg · L-1), rhFGF (5 mg · L-1) and recombinant human basic fibroblast growth factor -1) and bovine brain extract (bBE, lO mg · L-1) 0.2 ml respectively. The wound tissue was harvested at 5 days after injury to determine the cell cycle and DNA content of full-thickness skin tissue by flow cytometry. Results: The contents of SPF, PI and DNA in all groups were significantly higher than those in NS group (P <0.05). CONCLUSION: Recombinant human acidic fibroblast growth factor can promote the proliferation of wound tissue after microwave burns, promote wound healing and shorten wound healing time.