Proteomic analysis identifies translationally controlled tumor protein as a mediator of phosphatase

来源 :中华医学杂志(英文版) | 被引量 : 0次 | 上传用户:aurogon1
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Background Considerable evidence suggests that phosphatase of regenerating liver-3 (PRL-3) plays multiple roles in cancer metastasis; however,the molecular mechanisms remain largely unknown.The aim of this study was to identify proteins associated with PRL-3-promoted colon cancer metastasis,by comparative proteomic analysis.Methods Proteomes of human colon cancer LoVo cells transfected with PRL-3 gene (LoVo-PRL-3) or empty vector PAcGFP-C3 (LoVo-control) were compared using 2D gel electrophoresis.Proteins that varied significantly in concentration were selected and identified using mass spectrometry.Expression of translationally controlled tumor protein (TCTP) mRNA and protein in LoVo-PRL-3 and LoVo-control cells was detected by real-time PCR and West blotting.Small interfering RNA (siRNA) targeting TCTP was used for silencing TCTP expression in LoVo-PRL-3 cells.Functional significance of TCTP in PRL-3-promoted colon cancer cell proliferation,migration and invasion was investigated by Cell Counting Kit-8 assay and transwell chamber.Results Seventeen proteins displaying significant and reproducible differences between LoVo-PRL-3 and LoVo-control cells were identified.Ten proteins were upregulated and seven were downregulated in LoVo-pRL-3 cells when compared with LoVo-control cells.Eight identified proteins are associated with distinct steps of tumor metastasis:ubiquitin-like protein ISG15,interleukin-18,TCTP,serpin B5,annexin A3,macrophage-capping protein,ATP-dependent RNA helicase DDX3X,and cathepsin D.Real-time PCR and West blotting results showed that both TCTP mRNA and protein were significantly increased in LoVo-PRL-3 cells compared to LoVo-control cells.Transfection with TCTP siRNA significantly reduced the expression of both mRNA and protein levels of TCTP in LoVo-PRL-3 cells.Knockdown of TCTP by siRNA inhibited PRL-3-promoted proliferation,migration and invasion of LoVo-PRL-3 cells.Conclusion Our results imply that TCTP might be a mediator of PRL-3-promoted proliferation,migration and invasion of human colon cancer cells.
其他文献
1 概况rn福建省大田县岩城水泥有限责任公司与合肥水泥研究设计院共同研究开发的国产600t/d五级旋风预热器回转窑采用无烟煤煅烧熟料,工程总投资1.2亿元,1998年12月12日建成
湖南省冶金材料研究所经过多年努力,研制成功一种具有广阔工业应用前景的新型高效耐磨材料--TiC 高锰钢复合材料,它的耐磨性能比目前国内外优良工业耐磨材料高出10倍,并于199
目的探讨早期乳腺癌行保乳手术的疗效。方法回顾性分析12例乳腺癌患者行保乳手术,术后化疗、放疗、内分泌治疗。结果本组患者近期均无瘤生存,局部无复发,远处无转移。结论在
目的观察针刺加走罐治疗腰椎间盘突出症的效果。方法采取针刺加走罐的方法对102例腰椎间盘突出症的患者进行治疗。结果治疗1个月后评定疗效:优80例,占80%;良12例,占12%;可5例,
电弧丝材喷涂技术始于欧美等发达国家,是80年代兴起的热喷涂技术中的新秀.湖南省冶金材料研究所经过多年探索、研究,在电弧丝材喷涂设备的研制方面取得了重大突破,成功地研制
“长城”系列粉末涂层是湖南省冶金材料研究所经过多年研究,开发的系列涂层产品.它的应用已涵盖机械、石油、化工、汽车、冶金等行业.
目的 旨在了解短期胰岛素强化治疗对初发的2型糖尿病胰岛功能的影响.方法 选择初发的2型糖尿病患者60例,对其肝肾功、空腹及餐后2 h血糖、胰岛素水平进行检测后随机分为两组:
Background Vascular endothelial growth factor (VEGF) is one of major mediators of angiogenesis and survival factor in some tissue,however,its direct effects on
研究了将玄武岩作为原料组分用于水泥生产的可能性及合理性.结果表明:将玄武岩以2.0%~3.4%的量用于原料混合物中,则可提高水泥生产的效率及产品的质量.
8.3 滑动轴承的间隙和油槽rn为使轴颈与轴瓦间形成一层油膜将二者金属表面隔开,避免直接摩擦磨损,在轴颈与轴瓦间必须具有一定的径向间隙;为了防止机器运转时因转轴与轴瓦热