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34例江苏启东地区原发性肝细胞性肝癌手术切除标本，分癌、癌旁（距癌肿ICM处）及癌外（距癌肿2CM以外）肝组织做HBVDNA点杂交和电泳转移杂交。结果：癌组织点杂交阳性率为58．5％（20／34）；癌旁肝组织阳性率为41．2％（14／34）；癌外肝组织阳性率为38．2％（13／34）。电泳转移杂交试验未见HBVDNA整合，癌、癌旁、癌外肝组织均显示游离的呈复制状态的HBVDNA。本实验表明：乙肝病毒DNA在肝细胞染色体基因组中的整合不是乙肝病毒导致肝癌发生的主要原因。
Thirty-four patients with primary hepatocellular carcinoma in Qidong, Jiangsu Province were treated with surgical specimens for HBV DNA hybridization and electrophoresis and transfer hybridization. The specimens were divided into cancer, adjacent to tumor (from cancerous ICM) and extracancer (2 cm away from cancer). RESULTS: The positive rate of spot hybridization in cancer tissue was 58.5% (20/34); the positive rate of para-carcinoma tissue was 41.2% (14/34); the positive rate of extra-cancerous liver tissue was 38.2% (13/ 34). No HBV DNA integration was observed in the electrophoretic transfer hybridization assay, and the replicated HBV DNA was displayed in cancer, paracancerous, and extracancerous liver tissues. This experiment shows that the integration of hepatitis B virus DNA in the chromosomal genome of liver cells is not the main cause of liver cancer caused by hepatitis B virus.