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目的 研究肝细胞生长因子 (hepatocyte growth factor,HGF)对视网膜色素上皮 (retinalpigment epitheliaum,RPE)细胞的促增生和促移行作用。 方法 在无血清培养液培养的人 RPE细胞中分别加入 1、2、10、5 0、10 0 μg/ L 不同浓度的 HGF,四唑盐比色法 (methyl thiazolyl tetrazolium,MTT)测定促细胞增生情况 ;采用 RPE细胞损伤愈合模型 ,在无血清培养液中分别加入 1、2、10、5 0、10 0μg/ L不同浓度的 HGF,培养 2 0 h后计数进入刮痕区的细胞 ,观察 HGF对 RPE细胞移行的作用。结果 HGF浓度处于10～ 10 0 μg/ L 时对 RPE细胞具有促增生作用 (P<0 .0 5或 P<0 .0 1) ,增生率为 18.2 %～ 34 .8% ,其中浓度为 5 0 μg/ L 作用 3d达到最大促增生效果 (P<0 .0 1) ,增生率为 32 .8% ;HGF可明显促进 RPE细胞移行 ,促移行能力分别为 113.0 % (10μg/ L ) ,91.7% (5 0μg/ L )和 5 0 .3% (10 0μg/ L )。浓度自 2μg/ L开始出现促细胞移行作用 (P<0 .0 5 ) ,促移行能力为 9.3%。结论 HGF可促进 RPE细胞增生和移行 ,是RPE细胞的有丝分裂原和强力的促移行因子。
Objective To investigate the effects of hepatocyte growth factor (HGF) on the proliferation and migration of retinal pigment epithelial cells (RPE). Methods Human RPE cells cultured in serum-free medium were treated with different concentrations of HGF (1, 2, 10, 50 and 100 μg / L, respectively) and methyl thiazolyl tetrazolium (MTT) In the RPE cell injury model, different concentrations of HGF were added into the serum-free medium at 1, 2, 10, 50 and 10 μg / L, respectively. After culturing for 20 h, the cells entering the scratch area were counted and HGF Effect on RPE cell migration. Results HGF at a concentration of 10 ~ 100 μg / L had a pro-proliferative effect on RPE cells (P <0.05 or P <0.01) with a proliferative rate of 18.2% -34.8%, with a concentration of 5 The effect of 0 μg / L treatment on RPE cells was most significant (P <0.01). The proliferation rate was 32.8%. HGF significantly promoted the migration and migration of RPE cells (113.0%, 91.7%, 91.7% % (50 μg / L) and 50.3% (100 μg / L). The concentration of 2μg / L began to appear cell migration (P <0. 05), to promote the ability to move 9.3%. Conclusion HGF can promote the proliferation and migration of RPE cells, which is a mitogenic and potent factor for migration of RPE cells.