论文部分内容阅读
目的:探讨TWEAK能否通过外泌体途径介导巨噬细胞对上皮性卵巢癌细胞转移的调控。方法:佛波酯诱导人单核细胞系THP-1为贴壁的巨噬细胞,TWEAK刺激巨噬细胞24h后抽提外泌体,透射电镜观察其形态;分别将PBS、巨噬细胞分泌的外泌体、TWEAK刺激后巨噬细胞分泌的外泌体与上皮性卵巢癌细胞系SKOV3、HO-8910pm共培养,48h后通过Transwell实验检测其迁移、侵袭能力。结果:透射电镜可见巨噬细胞分泌的外泌体呈圆形,直径30~100nm。Transwell实验显示,与空白对照组相比,巨噬细胞分泌的外泌体可使上皮性卵巢癌细胞的迁移和侵袭能力增高;而TWEAK刺激巨噬细胞后,其分泌的外泌体则可降低其迁移和侵袭能力。结论:TWEAK可通过外泌体途径逆转巨噬细胞对上皮性卵巢癌细胞的促转移作用。
OBJECTIVE: To investigate whether TWEAK can mediate the regulation of epithelial ovarian cancer cell metastasis by macrophages via exosomes pathway. METHODS: Phorbol ester induced human monocytic cell line THP-1 to adhere to macrophages. Exosomes were stimulated with TWEAK for 24 hours and their morphology was observed by transmission electron microscopy. The secretion of PBS and macrophages Exosomes and exosomes secreted by macrophages after TWEAK stimulation were co-cultured with epithelial ovarian cancer cell lines SKOV3 and HO-8910pm. After 48h, the migration and invasion were evaluated by Transwell assay. Results: Transmission electron microscopy showed macrophages secreted exosomes were round, diameter 30 ~ 100nm. Transwell experiments showed that compared with the control group, exosomes secreted by macrophages increased the migration and invasion ability of epithelial ovarian cancer cells. However, excretion of exosomes after TWEAK stimulation of macrophages decreased Its ability to migrate and invade. CONCLUSION: TWEAK reverses the pro-metastatic effect of macrophages on epithelial ovarian cancer cells via exosomes.