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[Celis E等:J Immunol 1984 132(3):1511(英文)] 10名接受了3次乙肝疫苗的供血者,在末次注射后的第7天采血100ml,用Ficoll密度梯度离心分离外周血单个核细胞(PBM),5×10~5/ml的PBM加入25ml含20μg/mlHBsAg和10%人AB血清的完全培液中,在37℃含5%CO_2的环境中孵育6天后再用
[Celis E et al .: J Immunol 1984 132 (3): 1511 (English)] Ten blood donors who received three Hepatitis B vaccinations and 100 ml of blood on day 7 after the last injection were centrifuged to separate peripheral blood cells by Ficoll density gradient (PBM), 5 × 10 ~ 5 / ml of PBM was added to 25ml of complete culture medium containing 20μg / ml HBsAg and 10% human AB serum and incubated for 6 days at 37 ℃ with 5% CO 2