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目的应用细胞因子抗体芯片技术分析急性脑梗死患者不同时段血清中细胞因子表达的变化。方法收集急性脑梗死40例,按不同发病时段分为发病2 h组、4 h组、6 h组、24 h组及72 h组,每组8例;另外,健康对照组8例。采用细胞因子抗体芯片技术平台,测定血清中60种细胞因子。结果与健康对照组比较脑梗死组患者血中细胞因子表达谱发生了明显变化:1有15种细胞因子表达水平普遍呈现为高表达,包括:IL-1β、IL-1ra、IL-2、IL-5、IL-6、IL-7、IL-10、IL-15、IL-16、GM-CSF、M-CSF、TNF-α、LIGHT、GDNF、Leptin等。其中,有部分在脑梗死发病早期表达高于后期,包括:IL-6、IL-7、IL-10;另有一部分在后期表达高于早期,包括:IL-5、GM-CSF;还有部分在脑梗死的某时段特别高,包括:IL-5、IL-3、IL-15、MCP-4;2有9种细胞因子表达水平普遍呈现为低表达,包括:BMP-4、Eotaxin-3、IGFBP-3、IGFBP-4、GCP-2、I-309、MCP-1、RANTES、CNTF。结论细胞因子可能直接或间接参与炎症细胞的活化和浸润,并在神经元的损伤与修复过程中起重要作用。
Objective To analyze the changes of serum cytokines in different periods of patients with acute cerebral infarction by using cytokine antibody chip technique. Methods 40 cases of acute cerebral infarction were collected and divided into 2 h, 4 h, 6 h, 24 h and 72 h groups according to the different stages of onset. There were 8 cases in each group. Using cytokine antibody chip technology platform, determination of serum 60 kinds of cytokines. Results Compared with the healthy control group, there was a significant change in the blood cytokine expression profiles in the patients with cerebral infarction: 1 The expression levels of 15 cytokines were generally high, including IL-1β, IL-1ra, IL-2, IL -5, IL-6, IL-7, IL-10, IL-15, IL-16, GM-CSF, M-CSF, TNF- alpha, LIGHT, GDNF, Leptin and the like. Among them, some of them were higher in early stage of cerebral infarction than those in late stage, including IL-6, IL-7 and IL-10; some were higher in early stage than early stage, including IL-5 and GM- Some of them were particularly high in a certain period of cerebral infarction, including IL-5, IL-3, IL-15 and MCP-4. The expression of 9 cytokines was generally low, including BMP-4, Eotaxin- 3, IGFBP-3, IGFBP-4, GCP-2, I-309, MCP-1, RANTES, CNTF. Conclusions Cytokines may be directly or indirectly involved in the activation and infiltration of inflammatory cells and play an important role in the process of neuronal injury and repair.