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目的探讨纳米氧化锌诱导血管内皮细胞(ECV304)凋亡与活性氧(ROS)的关系。方法采用龙胆紫染色观察细胞吞噬效应;四甲基偶氮噻唑蓝(MTT)法测定细胞生长活性;琼脂糖凝胶电泳检测细胞凋亡;紫外分光光度计测定超氧化物歧化酶(SOD)、丙二醛(MDA)的含量;荧光分光光度计测定ROS。结果 ECV304细胞可吞噬纳米氧化锌,实验组MTT值(0.85±0.04)、(0.56±0.03)、(0.51±0.05)、(0.45±0.044)与对照组(1.21±0.11)比较,差异有统计学意义(P<0.01);实验组ROS(33.14±0.38)、(35.82±0.61)、(54.13±0.24)、(68.81±1.24)均高于对照组(30.12±1.23);实验组SOD(25.72±0.48)、(25.64±0.36)、(25.53±0.36)、(25.25±0.44)均低于对照组(26.39±0.40);实验组MDA含量均高于对照组(0.56±0.01);a-硫辛酸组细胞凋亡率明显降低。结论纳米氧化锌可以抑制ECV304细胞的活性,通过ROS途径诱导细胞凋亡,产生细胞毒性。
Objective To investigate the relationship between apoptosis induced by nano zinc oxide and reactive oxygen species (ROS) in vascular endothelial cells (ECV304). Methods Phagocytosis was observed by gentian violet staining, cell growth activity was measured by MTT assay, apoptosis was detected by agarose gel electrophoresis, and superoxide dismutase (SOD) , Malondialdehyde (MDA) content; fluorescence spectrophotometer ROS. Results The ECV304 cells could engulf the nano-ZnO. The MTT values in the experimental group were (0.85 ± 0.04), (0.56 ± 0.03), (0.51 ± 0.05), (0.45 ± 0.044) and the control group (1.21 ± 0.11) (P <0.01). The levels of ROS (33.14 ± 0.38), (35.82 ± 0.61), (54.13 ± 0.24) and (68.81 ± 1.24) in the experimental group were significantly higher than those in the control group (30.12 ± 1.23) (25.64 ± 0.36), (25.53 ± 0.36) and (25.25 ± 0.44) in the experimental group were significantly lower than those in the control group (26.39 ± 0.40) Group apoptosis rate was significantly reduced. Conclusion Nano-ZnO can inhibit the activity of ECV304 cells and induce apoptosis through ROS pathway, resulting in cytotoxicity.