论文部分内容阅读
目的研究食源性腹泻儿童肠道菌群的变化,为儿童食源性腹泻的防治提供理论依据。方法以10例食源性腹泻儿童粪便样本及10例健康儿童的粪便样本为研究对象,提取粪便总DNA,利用细菌16S rDNA V3区通用引物进行PCR扩增,扩增后产物进行变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)分析,并用Quantity One软件进行分析。结果食源性腹泻组儿童和对照组健康儿童的肠道菌群多样性存在显著性差异,食源性腹泻组的多样性低于对照组。食源性腹泻组儿童肠道菌群具有较高的相似性并与对照组儿童肠道菌群能聚类到不同簇中。结论食源性腹泻儿童肠道菌群多样性发生明显变化,说明肠道菌群的多样性调控可以应用于食源性腹泻的缓解或治愈。
Objective To study the changes of intestinal flora in children with food-borne diarrhea and provide theoretical basis for the prevention and treatment of food-borne diarrhea in children. Methods Stool samples of 10 children with food-borne diarrhea and 10 healthy children were used as the research object. Total DNA of the feces was extracted and PCR amplification was performed using the universal 16S rDNA V3 region primers. After amplification, the denatured gradient gel Denaturing gradient gel electrophoresis (DGGE) analysis was performed and analyzed using Quantity One software. Results There were significant differences in intestinal flora diversity between healthy children in food-borne diarrhea group and control group, while those in food-borne diarrhea group were lower than those in control group. The intestinal flora of children with food-borne diarrhea had higher similarity and clustered into different clusters with that of the control group. Conclusion The diversity of intestinal microflora in children with food-borne diarrhea significantly changes, indicating that the diversity of intestinal microflora can be used to alleviate or cure food-borne diarrhea.