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报道了中国北部沿海马粪海胆、海刺猬、光棘球海胆三种主要海胆与引自日本的中间球海胆四种海胆之间的不同组合的杂交试验及其子代浮游幼体及幼海胆的早期生长发育。结果表明 ,采用生殖调控可使不同海胆达到同步繁殖 ,在 8～ 2 4℃下各种海胆杂交组合的受精率与亲本亲缘关系有关 ,同时受到双亲繁殖适宜温度的影响 ,受精率介于 0～ 6 9.6 %之间 ,均低于自交组。以马粪海胆和海刺猬为亲本的正反交、以马粪海胆和中间球海胆为亲本的正反交以及马粪海胆 (♀ )×光棘球海胆 (♂ )各组杂交胚胎均可发育至四腕幼虫 ,但畸形率较高 ,浮游时间比自交组延长 3～ 1 0d ,在其中的 3个杂交组得到了幼海胆 ,经过 1 4个月的室内培育 ,杂交组成活率低于自交组 ,壳直径达 2 .5 9～ 2 .88cm
This paper reports the hybridization experiments of different combinations of three kinds of sea urchins, namely, sea urchin, coastal hedgehog and echinochloa crus-galli, and four species of sea urchin, introduced from Japan, and the early stage of their young offspring larvae and young sea urchins Growth and development. The results showed that different sea urchins could be reproduced synchronously by adopting reproductive regulation. The fertilization rate of various sea urchin hybrid combinations was related to the parental relationship at 8 ~ 24 ℃, and the fertilization rate was between 0 ~ 6 9.6%, lower than the selfing group. Reciprocal crosses between male and female sea urchins and sea hedgehog were carried out. The reciprocal crosses of both male and female sea urchins, and the hybrid embryos of each group of sea urchin, To four larvae, but the rate of deformity was higher. The lag time was 3 ~ 10 days longer than the selfing group, and the juvenile sea urchins were obtained in 3 of them. After 14 months of in vitro culture, the survival rate of hybrids was lower than Selfing group, shell diameter of 2.59 ~ 2.88cm