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采用EPR波谱仪测定肺组织内自由基变化以评价快速减压对肺的损伤。把SD大鼠在0.5MPa空气中暴露60min,用1min匀速减至常压,然后分别在45min和90min时腹腔放血处死(处死前25min注入0.05M的PBN,0.5ml/100g),取肺0.75g匀浆后用EPR波谱仪测定维生素C自由基(Vc)强度。结果显示减压后45min肺组织内Vc强度无明显变化,减压90min组的肺组织内Vc强度明显增高(P<0.05)。表明快速减压可引起肺组织自由基增加。
EPR spectroscopy was used to measure the change of free radicals in lung tissue to evaluate the lung injury caused by rapid decompression. The SD rats were exposed to air at 0.5MPa for 60min and then reduced to normal pressure in 1min. Then they were sacrificed at 45min and 90min respectively. Peripheral blood was excreted (0.05M PBN, 0.5ml / 100g) After homogenized with 0.75g EPR spectrometer determination of vitamin C radical (Vc) intensity. The results showed that there was no significant change of Vc intensity in lung tissue at 45 min after depressurization. Vc intensity in lung tissue of 90 min decompression group was significantly increased (P <0.05). It shows that rapid decompression can cause the increase of free radicals in lung tissue.