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为了鉴别免疫猪群3ABC抗体阳性猪是免疫动物受到病毒感染引起的,还是疫苗中残留的非结构蛋白引起的,通过检测免疫动物体内的3ABC抗体和病毒感染情况,探讨3ABC抗体的出现与免疫剂量的关系。选取60日龄保育猪20头,分成A、B、C、D 4个组,分别注射猪口蹄疫O型灭活疫苗(OS/99株)1、2、4、6m L/头。每组猪分别在免疫前1 d以及免疫后3、7、14、30和60 d,采集血清和鼻咽拭子,使用口蹄疫3ABC间接ELISA检测试剂盒、口蹄疫多重RT-PCR检测试剂盒以及病毒分离试验,检测3ABC抗体和病毒感染情况,发现保育猪在低剂量(1 m L/头或2 m L/头)免疫后没有检测到非结构蛋白抗体,而高剂量(4 m L/头或6m L/头)免疫后出现非结构蛋白抗体阳性率升高,而且出现非结构蛋白抗体阳性的动物中没有检出病毒感染或病毒核酸。检测结果提示这些免疫猪群非结构蛋白抗体的形成与免疫剂量有关。
In order to identify 3ABC-positive pigs in immunized pigs were caused by virus infection or non-structural protein residues in vaccinated animals, the detection of 3ABC antibody and virus infection in immunized animals was used to investigate the occurrence of 3ABC antibody and immunological dose Relationship. The 60-day-old nursery pigs were selected and divided into four groups (A, B, C and D). The animals were injected with 1, 2, 4, and 6 m L of O / Serum and nasopharyngeal swabs were collected at 1 day before immunization and at 3, 7, 14, 30 and 60 days after immunization. Indirect ELISA assay kit for foot-and-mouth disease (3ABC), multi-RT-PCR assay kit In a separate assay to detect 3ABC antibody and virus infection, it was found that non-structural protein antibodies were detected in the nursery pigs after immunization at low doses (1 m L / head or 2 m L / head), whereas high-dose (4 m L / 6m L / head) showed an increased positive rate of non-structural protein antibodies, and no virus or viral nucleic acid was detected in non-structural protein antibody-positive animals. The results suggest that the formation of these non-structural protein antibodies in immunized pigs is related to the dose of immunization.