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目的 观察人伤寒沙门菌Vi抗原对大肠杆菌菌毛抗原装配的影响。方法 利用体内、外同源重组系统 ,构建了VipR基因缺失突变的人伤寒沙门菌菌株 ,导致其Vi抗原的表达较相应野生菌株偏低。用包含产肠毒素大肠杆菌菌毛抗原基因的表达质粒分别转化Vi表达弱化菌株和相应野生菌株 ,对两者表达的菌毛抗原进行含量分析。结果 产肠毒素大肠杆菌CS3、CFA Ⅰ在VipR突变体菌株表面的含量 ,均比在相应野生菌株表面的含量高。结论 Vi抗原的表达弱化可能有利于菌毛抗原在人伤寒沙门菌表面的装配。本研究结果对于产肠毒素大肠杆菌基因工程疫苗的构建有指导意义。
Objective To observe the effect of Salmonella typhi Vi antigen on the assembly of E. coli pilus antigen. Methods Salmonella typhimurium strain with deletion of VipR gene was constructed by in vitro and in vivo homologous recombination system, resulting in lower expression of Vi antigen than the corresponding wild strain. The expression plasmid containing the enterotoxigenic Escherichia coli pilus antigen gene was used to transform the attenuated Vi expression strain and the corresponding wild strain respectively, and the content of the pili antigen expressed by the two strains was analyzed. Results The contents of enterotoxigenic Escherichia coli CS3 and CFA I on the surface of VipR mutant strain were higher than those on the corresponding wild strain. Conclusion The weakening of the expression of Vi antigen may be beneficial for the assembly of pili antigens on the surface of Salmonella typhi. The results of this study for the construction of enterotoxigenic Escherichia coli genetically engineered vaccine has guiding significance.