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目的 研究硒对大鼠海马神经细胞生长、存活及突起发育的影响。方法 利用新生大鼠海马神经细胞原代培养技术 ,培养液内加入不同浓度的硒 (6 2 5、12 5 0、187 5 μg/L)和碘加硒 ,观察有血清和无血清条件培养下海马神经细胞的生长、存活数 ;同时还测量了接种后 4个不同观测点的平均最长突起长度。结果 硒不仅对海马神经细胞早期突起生长有显著的促进作用 ,在接种 16、2 4、36、4 8h后有明显突起伸长趋势 ,各硒组与对照组相比平均增加 15～ 2 0 μm ,而且无论有无血清 ,硒均能延长神经细胞存活时间 ,提高存活率。结论 硒对海马神经细胞的早期突起生长、存活有重要作用。
Objective To study the effects of selenium on the growth, survival and neurite outgrowth of hippocampal neurons in rats. Methods Primary cultured hippocampal neurons of neonatal rats were cultured in vitro. Selenium (6 2 5,12 5 0,187 5 μg / L) and iodine plus selenium were added into the culture medium under the condition of serum and serum-free culture The growth and survival of hippocampal neurons were also measured. At the same time, the average longest protrusion length of 4 different observation points after inoculation was also measured. Results Selenium not only promoted the early neurite outgrowth of hippocampus, but also showed obvious protracted prolapse after 16, 24, 36, 48 h of inoculation. The selenium group increased 15 ~ 20 μm on average compared with the control group , And whether or not serum, selenium can prolong the survival time of nerve cells and improve the survival rate. Conclusion Selenium plays an important role in the early process of neurite outgrowth and survival in hippocampus.