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目的研究羊栖菜多糖对氧化应激的人皮肤成纤维细胞的保护作用及机制。方法以0.8 mmol/L H2O2诱导人皮肤成纤维细胞建立氧化应激模型,MTT法测定不同浓度羊栖菜多糖(15、30、60mg/L)对过氧化氢(H2O2)损伤人皮肤成纤维细胞增殖的影响,Hoechst 33258染色法观察细胞凋亡,流式细胞仪测定细胞周期分布。测定细胞丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、还原性谷胱甘肽(GSH)水平和活性氧(ROS)水平。结果羊栖菜多糖可抑制H2O2对人皮肤成纤维细胞增殖活性的下降和凋亡率的上升,维持细胞正常形态。流式细胞仪检测表明,羊栖菜多糖能显著降低H2O2损伤的细胞G0/G1期阻滞现象;升高SOD和GSH-Px活力,降低MDA和ROS水平,升高GSH水平,差异有统计学意义且具有剂量依赖性(P<0.05,P<0.01)。结论羊栖菜多糖能减轻H2O2对人皮肤成纤维细胞的氧化损伤,具有较好的抗氧化保护作用,与其增强抗氧化酶活性,降低脂质过氧化有关。
Aim To study the protective effect of polysacchrides of Sargassum fusiforme against oxidative stress on human skin fibroblasts and its mechanism. Methods Oxidative stress was induced by 0.8 mmol / L H2O2 in human dermal fibroblasts. MTT was used to determine the effects of different concentration of polysaccharides (15,30 and 60 mg / L) on hydrogen peroxide (H2O2) -induced human skin fibroblasts Proliferation, Hoechst 33258 staining was used to observe the apoptosis, and the flow cytometry was used to determine the cell cycle distribution. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH) and reactive oxygen species Level. Results Sargassum polysaccharide can inhibit H2O2 on human dermal fibroblast proliferation activity decreased and the rate of apoptosis increased to maintain normal cell morphology. Flow cytometry showed that the polysaccharides from Sargassum fusiforme significantly reduced the G0 / G1 phase arrest induced by H2O2, increased the activities of SOD and GSH-Px, decreased the levels of MDA and ROS, and increased the levels of GSH Significance and dose-dependent (P <0.05, P <0.01). Conclusion The polysaccharides of Sargassum fusiforme can alleviate the oxidative damage of human skin fibroblasts induced by H2O2, and have good antioxidative protective effect, which is related to the enhancement of antioxidant enzyme activity and lipid peroxidation.