论文部分内容阅读
目的:观察红景天对心肌梗死大鼠心肌组织肾上腺髓质素(AM)及其受体降钙素受体样受体(CRLR)表达的影响,探讨红景天促进大鼠缺血心肌血管新生的可能机制,了解红景天苷是否为红景天该作用的有效成分。方法:建立Wistar大鼠急性心肌梗死模型,造模前7d开始分组灌胃生理盐水、红景天或红景天苷,灌胃持续至造模手术后第7天,处死动物取心脏标本,进行HE染色,免疫组化测定梗死心肌边缘区和非梗死区CD31阳性反应强度,实时定量聚合酶链式反应法和蛋白印迹法检测心肌组织AM、CRLR的表达。结果:红景天组梗死边缘区心肌CD31表达显著高于生理盐水组(P<0.05);红景天组心肌组织AM、CRLR表达均明显高于生理盐水组(P<0.05),红景天苷组各指标的表达水平均介于生理盐水组和红景天组之间。结论:红景天具有上调AM、CRLR表达和促进AMI大鼠缺血心肌血管新生的作用,红景天苷是红景天发挥该作用的有效成分之一。
Objective: To observe the effects of Rhodiola on the expression of adrenomedullin (AM) and its receptor calcitonin receptor-like receptor (CRLR) in myocardial tissue of rats with myocardial infarction, and to explore the effects of Rhodiola on ischemic myocardial blood vessels Newborn possible mechanism to understand whether salidroside Rhodiola is the active ingredient. Methods: Acute myocardial infarction (AMI) model of Wistar rats was established. Group 7 received normal saline, Rhodiola salidroside or salidroside for 7 days before model making. The expression of AM and CRLR in myocardium was detected by HE staining and immunohistochemistry. The CD31 positive reaction intensity was detected by immunohistochemistry in myocardium and non-infarcted area. The expression of AM and CRLR in myocardium was detected by real-time quantitative polymerase chain reaction and Western blot. Results: The expression of CD31 in myocardium of Rhodiola rosea group was significantly higher than that of saline group (P <0.05). The expression of AM and CRLR in Rhodiola rosea group was significantly higher than that of saline group (P <0.05) Glucosidase expression of each index were between the saline group and Rhodiola group. CONCLUSION: Rhodiola has the effects of up-regulating AM and CRLR expression and promoting angiogenesis in ischemic myocardium of AMI rats. Salidroside is one of the effective components of this effect.