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报告了埃及民间用药的蛋白酶抑制作用,以及印度尼西亚产的桃花心木(Swieteniamabagoni)树皮中该酶抑制活性成分的分离。制备41种埃及民间用药的甲醇、水提取物,探讨了对从大肠菌中发现的重组HIV蛋白酶的抑制作用。反应混合物(全量10μl)包含以下物质;蛋白酶、合成肽组氨酸-赖氨酸-丙氨酸-精氨酸-缬氨酸-亮氨酸-pNO_2-苯丙氨酸)-谷氨酸-丙氨酸-Nle-丝氨酸-NH_2(2mg/
The inhibition of proteases in Egyptian folk medicines was reported, as well as the isolation of the enzyme-inhibitory active ingredients in the bark of Swieteni amabagoni produced in Indonesia. The methanol and water extracts of 41 Egyptian folk medicines were prepared and the inhibitory effect of the recombinant HIV protease found in E. coli was explored. The reaction mixture (full volume 10 [mu]l) contains the following; protease, synthetic peptide histidine-lysine-alanine-arginine-valine-leucine-pNO2-phenylalanine)-glutamic acid- Alanine-Nle-serine-NH 2 (2mg/