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目的探讨同种异体皮质骨和松质骨愈合方式的差异。方法兔左侧尺骨中段植人冻干异体骨，分为皮质骨组和松质骨组，均为32只。术后2、4、8、16周分批处死，取材进行Ⅰ型胶原基因的原位杂交研究，以及皮质骨孔隙率和松质骨平均小梁宽度的组织学定量分析。结果异体皮质骨内先出现大量的骨吸收陷窝，然后在陷窝内出现表达1型胶原mRNA的成骨细胞；其孔隙率先增大，然后缓慢减小。异体松质骨内早期就有大量表达五型胶原mRNA的成骨细胞沉积在原有骨小梁表面，附近同时有破骨细胞的活动；其平均小梁宽度持续上升。结论同种异体皮质骨的愈合是在吸收的基础上成骨，而同种异体松质骨内则可以直接成骨。
Objective To investigate the differences of healing methods between allogeneic cortical bone and cancellous bone. Methods Rabbit allogeneic rabbits were randomly divided into three groups: cortical bone group and cancellous bone group, all of which were 32. The rats were sacrificed at 2, 4, 8, and 16 weeks after operation. In situ hybridization of type I collagen was performed and histological and quantitative analysis of cortical bone porosity and average trabecular width of cancellous bone were performed. Results A large number of bone resorption lacunae were found in allogeneic cortical bone. The osteoblasts expressing type 1 collagen mRNA appeared in the lacunae. The porosity increased first and then slowly decreased. Early in allogeneic cancellous bone, a large number of osteoblasts expressing collagen type V mRNA were deposited on the surface of the original trabecular bone with osteoclast activity in the vicinity. The average trabecular width continued to increase. Conclusion The healing of allogeneic cortical bone is osteogenesis on the basis of absorption, while allogeneic cancellous bone can be directly osteogenesis.