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目的探讨白细胞介素(IL) 18 抗体干预,了解IL 18 抗体对急性免疫性肝坏死动物模型的影响。方法取BALB/c 小鼠18 只,随机平分为三组A组(正常对照组);采用结核菌苗(BCG)加脂多糖(LPS)诱导法建立急性免疫性肝损伤模型B组(单纯病理组);抗体治疗组(C组)在模型形成前用IL 18抗体干预。实验结束取所有BALB/c小鼠肝组织,行病理学肝组织炎症活动度计分(HAI),血清IL 18由ELISA法检测,肝组织IL 18、肿瘤坏死因子α(INF α)、干扰素γ(IFN γ)由RT PCR检测。结果C组HAI 3 333±1 633,明显低于B组19 000±0 548(P<0 05);B组血清IL 18明显较C组增高(P<0 05);B组肝组织IL 18、IFN γ、INF αmRNA表达水平明显较C组增高(P<0 05)。结论IL 18、IFN γ、TNF α在急性免疫性肝坏死动物模型的形成中发挥重要作用,IL 18抗体可干预其形成。
Objective To investigate the intervention of interleukin (IL) 18 antibody to understand the effect of IL-18 antibody on acute immune hepatic necrosis in animal models. Methods Eighteen BALB / c mice were randomly divided into three groups A (normal control group). Acute immunological liver injury model group B (simple pathology) was established by using the method of BCG plus lipopolysaccharide (LPS) induction. Group). Antibody treatment group (group C) was treated with IL-18 antibody before model formation. At the end of the experiment, all the BALB / c mouse liver tissues were taken for histopathological liver activity score (HAI), serum IL-18 was detected by ELISA, liver tissue IL-18, tumor necrosis factor alpha (INF alpha) γ (IFN γ) was detected by RT PCR. Results The HAI of group C was 333 333 ± 1 633, which was significantly lower than that of group B 19 000 ± 0 548 (P <0.05). The serum IL 18 of group B was significantly higher than that of group C (P <0.05) , IFNγ, INFαmRNA expression levels were significantly higher than those in C group (P <0.05). Conclusion IL 18, IFN γ, TNF α play an important role in the formation of animal model of acute immune hepatic necrosis. Interleukin-18 antibody can interfere with the formation of the animal model.