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目的探讨大豆异黄酮SI-I抑制HeLa细胞生长机制。方法 HeLa细胞经过10,20,and 40 g/ml的大豆异黄酮SI-I处理4d后,采用倒置显微镜和透射电镜观察其形态学变化,利用流式细胞仪检测细胞周期分布,通过激光扫描共聚焦显微镜观察细胞中[Ca2+]i浓度。结果倒置显微镜观察到HeLa细胞数量明显变少,细胞严重变形。透射电镜观察到HeLa细胞发生凋亡形态学变化。流式细胞仪分析发现HeLa细胞周期阻滞于G0/G1或G2/M期,细胞凋亡率随SI-I浓度的增加而上升。激光扫描共聚焦显微镜观察到凋亡的HeLa细胞内[Ca2+]i显著升高。结论大豆异黄酮SI-I抑制HeLa细胞生长是通过凋亡诱导作用,并且细胞周期阻滞和[Ca2+]i浓度升高可能是其诱导凋亡机制之一。[营养学报,2012,34(4):373-378]
Objective To investigate the mechanism of soybean isoflavone SI-I inhibiting HeLa cell growth. Methods HeLa cells were treated with 10, 20, 40 g / ml SI-I for 4 days. The morphological changes of HeLa cells were observed by inverted microscope and transmission electron microscope. Cell cycle distribution was detected by flow cytometry. The concentration of [Ca2 +] i in the cells was observed under a confocal microscope. Results Inverted microscope showed that the number of HeLa cells decreased significantly and the cells were severely deformed. The morphological changes of apoptosis in HeLa cells were observed by transmission electron microscope. Flow cytometry analysis showed that cell cycle of HeLa cells was arrested in G0 / G1 or G2 / M phase, and apoptosis rate increased with the increase of SI-I concentration. Laser scanning confocal microscopy showed a significant increase of [Ca2 +] i in apoptotic HeLa cells. Conclusion Soy isoflavone SI-I inhibits the growth of HeLa cells through the induction of apoptosis, and cell cycle arrest and the increase of [Ca2 +] i concentration may be one of the mechanisms of apoptosis induction. [Journal of Nutrition, 2012,34 (4): 373-378]