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探索了电子嗅传感仪直接通过发酵尾气进行发酵液中甲醇浓度在线检测的方法,建立了毕赤酵母表达糖化酶过程中甲醇浓度的自动化反馈补料控制模型,可准确实现发酵过程中甲醇浓度的精确控制;研究表明,当利用电子嗅将培养液中甲醇浓度稳定控制在(890±35)ppm水平下,发酵诱导培养到128h时目的蛋白糖化酶酶活达到了8 153U/ml,与甲醇浓度控制在(350±26)ppm时的发酵水平相比提升了48.8%。该方法具有无需前处理、与发酵液非接触、快速和准确性的优点,为提升工程酵母在工业发酵培养过程工艺的优化控制具有重要的指导作用。
The method of on-line detection of methanol concentration in fermentation broth directly by the fermentation exhaust gas was explored. An automatic feed-back control model of methanol concentration in the expression of pectinase in Pichia pastoris was established. The methanol concentration The results showed that when the concentration of methanol in culture medium was controlled at (890 ± 35) ppm level by electronic sniffing, the activity of saccharifying enzyme of target protein reached 8 153U / ml when it was induced to 128h by fermentation, The fermentation level increased by 48.8% when the concentration was controlled at (350 ± 26) ppm. The method has the advantages of no pretreatment, non-contact with the fermentation broth, rapidity and accuracy, and plays an important guiding role in optimizing the process control of the engineering yeast in the industrial fermentation process.