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目的:筛选并探讨胃癌相关激肽释放酶(kallikrein,KLK)在胃癌中的表达,及其与胃癌临床病理和预后的关系。方法:用cDNA微阵列检测胃癌组织及正常胃黏膜标本,筛选出在胃癌组织中差异表达的KLK;用real-timePCR方法进一步验证;再用免疫组化检测胃癌组织石蜡切片中之目标KLK表达水平,并分析其与胃癌病人临床特征及预后的关系。结果:胃癌组织中,KLK10表达上调9.55倍,KLK11表达下调12.02倍。KLK10的表达水平与肿瘤直径、肿瘤浸润深度及淋巴结转移情况明显相关,而KLK11的表达水平与这些临床病理学特征均无关;肿瘤浸润深度、淋巴结转移情况及KLK10和KLK11表达状况与病人的总体生存率明显相关。结论:胃癌组织中差异表达的KLK主要包括KLK10及KLK11;KLK10的表达水平与肿瘤直径、肿瘤浸润深度、淋巴结转移情况及病人的总体生存率明显相关;而KLK11的表达水平与这些临床病理学特征均无相关,而与病人的总体生存率明显相关。
Objective: To screen and investigate the expression of kallikrein (KLK) in gastric cancer and its relationship with clinicopathology and prognosis of gastric cancer. Methods: The gastric cancer tissues and normal gastric mucosa specimens were detected by cDNA microarray. KLKs differentially expressed in gastric cancer tissues were screened out. The expression of KLK in paraffin sections of gastric cancer tissues was further verified by real-time PCR. , And analyzed its relationship with clinical features and prognosis of gastric cancer patients. Results: In gastric cancer tissues, the expression of KLK10 was up-regulated 9.55-fold and KLK11 was down-regulated 12.02-fold. The expression level of KLK10 was significantly correlated with tumor diameter, depth of tumor invasion and lymph node metastasis, while the expression level of KLK11 was not related to these clinicopathological features. The depth of tumor invasion, lymph node metastasis and the overall survival of KLK10 and KLK11 The rate is obviously related. Conclusions: KLK differentially expressed in gastric cancer tissues mainly include KLK10 and KLK11. The expression level of KLK10 is significantly correlated with tumor diameter, depth of tumor invasion, lymph node metastasis and overall survival of patients. However, the expression of KLK11 is associated with these clinicopathological features Are not related, but significantly related to the overall survival of patients.