论文部分内容阅读
目的 研究腹腔镜结直肠癌手术对肿瘤切口种植及脏器转移的影响。方法 选用人结肠癌细胞 (Lo Vo细胞 )悬液 (1× 10 7/L) ,OT针注入雌性Balb/C裸鼠盲肠浆膜下 (0 1mL) ,建立人结肠癌细胞裸鼠原位种植模型。 2周后 ,实验裸鼠随机分为 3组 :CO2 人工气腹术组 (31只 )、剖腹术组 (31只 )、和未行手术组 (30只 )。 10周后 ,3组裸鼠均脱颈法处死 ,探查肿瘤细胞原位种植及致瘤鼠肿瘤细胞切口种植及各脏器转移情况。结果 (1) 3组裸鼠的致瘤率为 :CO2 人工气腹组 2 3/ 31例 ,开腹术组 2 2 / 31例 ,未手术组 2 1/ 30例 ,差异无显著意义。 (2 )CO2 人工气腹术组的 2 3只致瘤鼠中 ,有 2只发生切口种植 ,12只发生脏器转移 ;开腹术组的 2 2只致瘤鼠中 ,有 4只发生切口种植 ,13只发生脏器转移 ;未手术组 2 1只致瘤鼠中 ,有 10只发生脏器转移 ,致瘤裸鼠的切口种植及脏器转移率差异无显著意义。结论 CO2 人工气腹没有促进人结肠癌细胞裸鼠原位种植模型切口种植及脏器转移的发生 ,腹腔镜结直肠癌手术具有一定的安全性与可行性。
Objective To study the effect of laparoscopic colorectal surgery on tumor incision implantation and organ metastasis. Methods LoVo cells suspension (1 × 10 7 / L) was injected into the female Balb / C nude mice subcutaneously (0 1 mL). The colon cancer cells were implanted in situ model. Two weeks later, the experimental nude mice were randomly divided into three groups: CO2 pneumoperitoneum group (31), laparotomy group (31) and no surgery group (30). After 10 weeks, all three groups of nude mice were sacrificed by cervical dislocation. The in situ implantation of tumor cells and the incision of tumor-bearing mice were observed. Results (1) The tumorigenic rates of nude mice in three groups were as follows: 2 31 cases in the CO2 artificial pneumoperitoneum group, 2 of 31 cases in the open abdominal surgery group and 2 1/30 in the non-surgical group, the difference was not significant. (2) In the CO2 artificial pneumoperitoneum group, of the 23 tumorigenic mice, 2 incisions were implanted and 12 organs were transplanted. Among the 2 2 tumorigenic mice in the abdominal surgery group, 4 incisions In the non-operation group, only 10 of the 21 tumorigenic mice had organ metastasis. There was no significant difference in incision implantation and organ metastasis rate in nude mice. Conclusion CO2 artificial pneumoperitoneum does not promote incision implantation and organ metastasis of orthotopic implantation model of human colon cancer cells in nude mice. Laparoscopic colorectal cancer surgery has some safety and feasibility.