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目的:研究金桂外洗方对完全弗氏佐剂性炎症痛小鼠血清和局部前列腺素E2(PGE2)浓度的影响。方法:将体质量180~200g SPF级雌雄各20只KM小鼠在右后脚掌以完全弗氏佐剂法建立炎症模型,随机分为金桂外洗方外洗组(中药组)、扶他林溶液外洗组(西药组)、金桂外洗方+扶他林溶液外洗组(中西组)、造模组(阳性组)和空白组,1周后取小鼠血清和局部组织进行ELISA来测定PGE2.结果:1)血清PGE2浓度:阳性组>西药组>中药组>中西组>空白组,阳性组和西药组、中药组和中西组、中西组与空白组组间比较,组间差异有统计学意义(P<0.01)。中药组、西药组、中西组、阳性组和空白组血清PGE2浓度分别是(1.02±0.20)pg/mL,(1.11±0.17)pg/mL,(0.85±0.15)pg/mL,(1.65±0.11)pg/mL,(0.56±0.14)pg/mL.2)局部组织PGE2浓度:阳性组>西药组>中药组>中西组>空白组。中西组与空白组组间比较,组间差异无统计学意义(P>0.05);阳性组与西药组、西药组与和中药组、中药组和中西组组间比较,组间差异有统计学意义(P<0.01)。中药组、西药组、中西组、阳性组和空白组局部组织PGE2浓度分别是(130.19±17.00)pg/mL,(210.29±14.50)pg/mL,(65.79±17.30)pg/mL,(788.00±95.15)pg/mL,(37.77±7.48)pg/mL.结论:金桂外洗方发挥消炎止痛的作用途径是通过降低血清和局部组织PGE2的浓度来实现,较扶他林作用明显。
OBJECTIVE: To study the effects of Jinguiwuxian Recipe on serum and local prostaglandin E2 (PGE2) concentrations in mice with complete Freund’s adjuvant inflammatory pain. METHODS: Twenty male and female KM mice of 180 ~ 200g SPF were injected into the right hind paw respectively with complete Freund’s adjuvant to establish an inflammatory model. The rats were randomly divided into two groups: (Western medicine group), Jingui external washing group (n = 5), Furadan washing group (middle and western group), model group (positive group) and blank group. One week later, serum and local tissues of mice were taken for ELISA to determine PGE2 Results: 1) Serum PGE2 concentration: positive group> western medicine group> traditional Chinese medicine group> western group> blank group, positive group and western medicine group, traditional Chinese medicine group and western group, western group and blank group Significance (P <0.01). The concentrations of PGE2 in the TCM group, the western medicine group, the Western medicine group, the positive group and the blank group were (1.02 ± 0.20) pg / mL, (1.11 ± 0.17) pg / mL, ) pg / mL, (0.56 ± 0.14) pg / mL.2) Local tissue PGE2 concentration: positive group> western medicine group> Chinese medicine group> western group> blank group. There was no significant difference between the two groups (P> 0.05). There was significant difference between the two groups (P> 0.05); between positive group and Western medicine group, Western medicine group and traditional Chinese medicine group, traditional Chinese medicine group and western group, Significance (P <0.01). The concentrations of PGE2 in the local tissues of Chinese medicine group, western medicine group, western medicine group, positive group and blank group were (130.19 ± 17.00) pg / mL, (210.29 ± 14.50) pg / mL and 65.79 ± 17.30 pg / mL, 95.15) pg / mL, (37.77 ± 7.48) pg / mL.Conclusion: The anti-inflammatory and analgesic actions of Jin-Gui Wa-Xi-Shuo Prescription can be achieved by decreasing the concentrations of PGE2 in serum and local tissues,