论文部分内容阅读
目的:探讨海马微环境内异常Ca2+与局部脑血流(regional cerebral blood flow,rCBF)相互作用变化及其可能机制。方法:单泵等速微灌流系统分别行树鼩海马高、中、低Ca2+微灌流4 h,通过激光多普勒血流计测量海马CA1区rCBF含量;并用电镜观测海马血管内皮细胞的超微结构。微灌流Ca2+溶液后原位灌流尼莫地平,观测上述指标的变化。结果:树鼩微环境内随着Ca2+的增加海马rCBF逐渐降低(P<0.01),其中高浓度Ca2+微灌流后的rCBF(PU)最低(2.9±0.81,P<0.01),使用Ca2+通道拮抗剂尼莫地平后可显著升高rCBF,同时在电镜下可观察到血管内皮细胞的损伤有所减轻。结论:缺血时rCBF的降低会引起微环境内Ca2+降低,致使微环境异常;而微环境内Ca2+异常也可致rCBF降低。
Objective: To investigate the changes of the interaction between abnormal Ca2 + and regional cerebral blood flow (rCBF) in hippocampal microenvironment and its possible mechanism. Methods: Single-pump isokinetic microperfusion system was used to detect the content of rCBF in hippocampal CA1 region of hippocampus by high, medium and low Ca2 + micro-perfusion in hippocampus for 4 hours respectively. The ultrastructure of hippocampus vascular endothelial cells structure. Micro-perfusion of Ca2 + solution in situ perfusion of nimodipine observed changes in these indicators. Results: The rCBF of hippocampus decreased gradually with the increase of Ca2 + in the shrews (P <0.01). The rCBF (PU) of the hippocampus was the lowest (2.9 ± 0.81, P <0.01) after the Ca2 + After nimodipine can significantly increase rCBF, at the same time under the electron microscope can be observed in vascular endothelial cell damage has been reduced. CONCLUSION: The decrease of rCBF during ischemia can cause the decrease of Ca2 + in the microenvironment, resulting in abnormal microenvironment. However, abnormal Ca2 + in the microenvironment may also cause the decrease of rCBF.