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【目的】探明不同种类的植物对其根际土壤微生物数量分布与群落结构的影响。【方法】将微生物计数法与磷脂脂肪酸(PLFA)法相结合,分析比较麦积山景区3种典型裸子植物根际土壤微生物的数量分布和PLFA种类、含量及主成分结构。【结果】3种植物根际土壤微生物数量均以细菌最多,真菌最少;总PLFA含量以红豆杉[Taxus chinensis(Pilg)Rehder.]最高、种类最多,日本落叶松[Larix kaempferi(Lamb)Carriere.]最低,红豆杉与云杉(Picea asperata Mast)PLFA主要成分相似度高于日本落叶松;外来种日本落叶松无论微生物数量,还是种类以及PLFA结构组成与红豆杉、云杉均有较大的差异,多样性显著下降。【结论】与土著裸子植物相比,外来种日本落叶松能明显改变根际土壤微生物数量分布与群落结构。
【Objective】 The objective of this study was to investigate the effects of different plant species on the distribution of number of microbes in rhizosphere soil and community structure. 【Method】 Microbial count method was combined with phospholipid fatty acid (PLFA) method to analyze and compare the rhizosphere soil microbial population distribution, PLFA type, content and main component structure of three typical gymnosperms in Maijishan scenic area. 【Result】 The results showed that the total number of microbes in the rhizosphere soil of the three species of plants was the highest with the lowest number of fungi. The total PLFA content was the highest in Taxus chinensis (Pilg) Rehder. The species was Larix kaempferi (Lamb) Carriere. ], The similarity of the main components of PLFA between Taxus chinensis and Picea asperata Mast was higher than that of Japanese larch; the number of alien species of Larix kaempferi was higher than that of Taxus chinensis and Spruce no. Differences, diversity decreased significantly. 【Conclusion】 Compared with native gymnosperms, the alien species Larix principis-rupprechtii can significantly change the rhizosphere soil microbial quantity distribution and community structure.