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目的:探讨茵陈素（6,7-二甲基香豆素）对肺癌细胞增殖和细胞周期的影响。方法:应用光镜、四氮甲唑蓝（MTT）方法和流式细胞术分析茵陈素对肺癌细胞形态学、生长和细胞周期的影响。结果:茵陈素能抑制肺癌细胞的增殖,并呈剂量依赖性,以160μg/mL抑制作用最明显,抑制率达52.4％。细胞被阻滞于G0/G1期,不能进入S及G2/M期,增殖指数明显下降。结论:茵陈素在体外对肺癌细胞具有抑制作用,通过抑制DNA合成,将细胞阻滞于G0/G1期,从而抑制细胞增殖。
Objective: To investigate the effect of Yinchen Su (6,7-dimethylcoumarin) on the proliferation and cell cycle of lung cancer cells. Methods: The morphological, growth and cell cycle of lung cancer cells were analyzed by light microscopy, MTT assay and flow cytometry. Results: Yin-Chen Su inhibited the proliferation of lung cancer cells in a dose-dependent manner. The most obvious inhibition was 160 μg / mL, with an inhibition rate of 52.4%. Cells were arrested in G0 / G1 phase, can not enter S and G2 / M phase, proliferation index decreased significantly. CONCLUSION: Inosine can inhibit the proliferation of lung cancer cells in vitro and inhibit cell proliferation by inhibiting the DNA synthesis and arresting the cells in G0 / G1 phase.