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目的 探讨上皮型钙粘附蛋白 (E cadherin)在乳腺肿瘤增殖及转移中的临床意义。方法 选用 10例正常乳腺组织、2 8例乳腺良性肿瘤及 4 0例乳腺恶性肿瘤标本 ,以半定量反转录PCR(RT PCR)检测E cadherinmRNA的表达。结果 正常乳腺和乳腺良性肿瘤组织中E cadherin的表达 ,分别为 (85 .3± 15 .7) %和 (80 .1± 2 1.2 ) % ,二者无显著差异(P >0 .0 1) ;而在恶性肿瘤组织中 ,E cadherin的表达则明显下降 ,仅为 (2 8.4± 16 .0 ) % (有淋巴结转移 )和 (42 .5± 19.1) %(无淋巴结转移 ) ,且与肿瘤的分级相关。结论 E cadherin在乳腺恶性肿瘤中的表达明显降低 ,与肿瘤的组织学分级相关。E cadherin表达的缺失或低表达 ,可能是导致乳腺癌高转移的因素之一
Objective To investigate the clinical significance of E cadherin in the proliferation and metastasis of breast cancer. Methods Ten cases of normal breast tissue, 28 benign breast tumors and 40 cases of breast cancer were selected for detection of E cadherin mRNA by semi-quantitative reverse transcription PCR (RT-PCR). Results The expressions of E cadherin in normal breast and benign breast tumor tissues were (85.3 ± 15.7)% and (80.1 ± 1.2.2)%, respectively, with no significant difference (P> 0.05) , While the expression of E cadherin in malignant tumor tissues was significantly lower than that of tumor (2 8.4 ± 16.0%) (with lymph node metastasis) and (42.5 ± 19.1)% (without lymph node metastasis) Grading related. Conclusion The expression of E cadherin in breast neoplasm was significantly lower than that in the histological grade. E cadherin expression loss or low expression may be one of the factors leading to breast cancer metastasis