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目的:探讨人参皂苷Rg1经PEG修饰前后在大鼠离体胃中的稳定性。方法:取SD大鼠,禁食18 h后,随机分为Rg1组和PEG-Rg1组,准确吸取Rg1胃灌注液和PEG-Rg1胃灌注液注射到离体胃内,37℃恒温振荡,不同时间点取样,采用UPLC对样品中指标成分人参皂苷Rg1进行含量测定,观察比较人参皂苷Rg1与PEG-Rg1在离体胃中的稳定性差异。结果:Rg1在大鼠离体胃中的稳定性差,2 h时测得Rg1为26.8%,降解73.2%,PEG-Rg1在大鼠离体胃中的稳定性提高,2 h时测得Rg1仍有81.8%,仅降解18.2%。结论:PEG修饰之后可以提高人参皂苷Rg1在胃中的稳定性,可以改善修饰之前游离的人参皂苷Rg1在胃中容易降解,稳定性差等问题。
Objective: To investigate the stability of ginsenoside Rg1 in isolated rat stomach before and after PEG modification. Methods: SD rats were fasted for 18 h, then randomly divided into Rg1 group and PEG-Rg1 group. Rg1 gastric perfusate and PEG-Rg1 gastric perfusion fluid were injected into the isolated stomach accurately and oscillated at 37 ℃. At time point sampling, UPLC was used to determine the content of ginsenoside Rg1 in the sample. The stability of isolated ginsenoside Rg1 and PEG-Rg1 was compared. RESULTS: Rg1 was poorly isolated from the stomach of rats, with an Rg1 of 26.8% at 2 h and a 73.2% degradation. The stability of PEG-Rg1 in isolated rat stomach was improved. Rg1 was still measured at 2 h 81.8%, only 18.2% degradation. CONCLUSION: The modification of PEG can improve the stability of ginsenoside Rg1 in the stomach, and can improve the problem of free degradation of ginsenoside Rg1 in the stomach and poor stability before modification.