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选用Ｃ１８预处理柱分离脐血浆内源性类洋地黄物质（Ｅｎｄｏｇｅｎｏｕｓｄｉｇｉｔａｌｉｓ－ｌｉｋｅｓｕｂｓｔａｎｃｅ，ＥＤＬＳ），应用高效液相色谱分析法（ＨＰＬＣ）对其进行部分纯化，观察部分纯化的ＥＤＬＳ对红细胞摄取８６Ｒｂ的抑制作用，并比较其与哇巴因在高效液相色谱图上的特点。结果表明：脐血浆第二份ＨＰＬＣ洗脱液（滞留时间４～６ｍｉｎ）对红细胞摄取８６Ｒｂ具有强烈的抑制作用；其抑制率为（８９±３）％，明显高于其它洗脱物份别及孕妇、健康成人对照组血浆第二份洗脱物的抑制率［分别为（７３±５）％和（４３±３）％，Ｐ值均＜０．０１］；部分纯化的ＥＤＬＳ、哇巴因及两者混合物的滞留时间基本一致，提示ＥＤＬＳ与哇巴因的结构相似。
The endogenous digitalis-like substance (EDLS) of umbilical cord plasma was isolated by C18 pretreatment and partially purified by high performance liquid chromatography (HPLC). The inhibition of erythrocyte uptake 86Rb by partially purified EDLS was observed And compare its characteristics with ouabain on HPLC. The results showed that the second HPLC eluent of umbilical cord plasma (residence time 4 ~ 6min) strongly inhibited the uptake of 86Rb by erythrocytes, and its inhibitory rate was (89 ± 3)%, which was significantly higher than that of other eluates The inhibitory rates of the second plasma elution in pregnant women and healthy adults were (73 ± 5)% and (43 ± 3)%, respectively, P <0.01. The partially purified EDLS and ouabain And the residence time of the mixture was basically the same, suggesting that EDLS and ouabain structure similar.