谷氨酸（Ｇｌｕ）和γ－氨基丁酸（ＧＡＢＡ）等神经递质的代谢异常是诱发多种神经系统疾病的因素之一。由于神经递质在脑内的含量很低，改良建立了一种用ＨＰＬＣ荧光检测Ｇｌｕ、ＧＡＢＡ等４种神经递质的方法，以高丝氨酸为内标，流动相为磷酸缓冲液和甲醇，梯度洗脱２０ｍｉｎ，荧光检测。分离效果及线性关系良好，Ｇｌｕ、ＧＡＢＡ的相关系数分别为０．９９６９、０．９９９６；精密度ＣＶ批内３．４％，批间５．６％；其最小检测限分别为３．３ｐｍｏｌ、１．６ｐｍｏｌ Metabolic abnormalities in neurotransmitters such as glutamate (Glu) and gamma-aminobutyric acid (GABA) are among the factors that trigger many neurological disorders. Due to the low content of neurotransmitters in the brain, a method for the detection of four neurotransmitters such as Glu and GABA by HPLC fluorescence was developed. Using homoserine as internal standard, the mobile phase consisted of phosphate buffer and methanol, and the gradient Elution 20min, fluorescence detection. The correlation coefficients between Glu and GABA were 0.9969 and 0.9996, respectively. The precision was 3.4% in CV batch and 5.6% in batch. The minimum detection limits were 3.3 pmol, 1.6pmol