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实验动物为7周龄Wistar系雄性大鼠。从甘薯块根皮提取物的透析液得85％乙醇可溶粉末。将其溶于15％三氯乙酸溶液,离心分离,透析上清得组分1。乙醇可溶性粉末进行丁基-Toyopar疏水层析得组分2。进一步用苯基-Toyopal疏水层析得组分3。组分3经超滤得组分4及组分4A、4B。尾静脉给予这些供试品(分别为4、2、2、2、1、1mg/kg),24h后尾静脉采血,测定血糖及血中胰岛素值。组分4进行口服葡萄糖耐量试验(葡
The experimental animals were 7-week-old Wistar male rats. The 85% ethanol-soluble powder was obtained from the dialysate of the sweet potato root bark extract. This was dissolved in a 15% trichloroacetic acid solution, centrifuged, and the supernatant was dialyzed to give fraction 1. Ethanol-soluble powder was subjected to butyl-Toyopar hydrophobic chromatography to obtain fraction 2. The component 3 was further purified by hydrophobic chromatography using phenyl-Toyopal. Component 3 was ultrafiltered to give component 4 and components 4A, 4B. The samples were given to the tail vein (4, 2, 2, 2, and 1 mg/kg, respectively). Blood was collected from the tail vein 24 hours later, and blood glucose and blood insulin were measured. Component 4 for Oral Glucose Tolerance Test (Portuguese