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目的 探讨免疫低下时感染性肺炎是否存在肠源性感染。方法 将健康Wistar大鼠 48只随机分 4组 ,正常对照组、肺炎组、肺炎地米组、肺炎环磷组。后两组分别在建立肺炎模型前 10d分别连续腹腔注射地塞米松、环磷酰胺 ,每天 1次 ,第 11d同时建立绿脓杆菌肺炎模型。 2 4h后无菌操作下取出大鼠脏器、匀浆、细菌培养、菌落计数及细菌鉴定。结果 ①大鼠存活率 :对照组及肺炎组存活率 10 0 % ;肺炎地米组 40 % (6 / 15 ) ,肺炎环磷组 80 % (12 / 15 )。②脏器细菌培养阳性多见于肠系膜淋巴结、肝脏 ,细菌鉴定以革兰阴性杆菌为主。③细菌培养阳性率及菌落量 :肺炎地米组、肺炎环磷组的阳性率及菌落量明显高于对照组及肺炎组。结论 免疫低下时绿脓杆菌肺炎大鼠中存在肠源性感染。
Objective To investigate if there is intestinal infection in infectious pneumonia when immunocompromised. Methods Forty-eight healthy Wistar rats were randomly divided into 4 groups: normal control group, pneumonia group, pneumoldi group and pneumocystin group. The latter two groups were given intraperitoneal injection of dexamethasone and cyclophosphamide once a day, respectively, 10 days before the establishment of pneumonia model. The model of Pseudomonas aeruginosa pneumonia was established on the 11th day. 2 4h after aseptic removal of organs, homogenates, bacterial culture, colony count and bacterial identification. Results ① The survival rate of rats was 100% in the control group and pneumonia group, 40% (6/15) in the pneumolysin group and 80% (12/15) in the pneumocystin group. ② organ culture positive more common in mesenteric lymph nodes, liver, bacteria identified as gram-negative bacilli. ③ bacterial culture positive rate and the amount of colonies: pneumonia rice group, pneumocystin group positive rate and the colony was significantly higher than the control group and pneumonia group. Conclusions There is gut-derived infection in Pseudomonas aeruginosa pneumonia rats with low immunity.