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DNA的分子组成,对其双螺旋结构的变性有影响,一般GC含量高的DNA不易变性。硷基组成的研究为进一步分析DNA、制备探针、分子杂交等基因工程的研究提供基础。为此,我们对两型卫氏并殖吸虫(3np·w,2np·w)及怡乐村并殖吸虫(p·i)的硷基组成进行了检测。 一、虫体来源与接种 除怡乐村并殖吸虫囊蚴采自辽宁省营口县水源乡的蟛蜞外,其它操作同文献[王恩荣等:中国寄生虫学与寄生虫病杂志1991;9(1):46]。 二、染色体DNA的制备 按Leornard所述方法,提取DNA。经测定OD260nm/OD280nm比值分别为1.65、1.73与1.75;DNA含量介于1.7～2.3μg/μl之间。含量与纯度达实验要求。
The molecular composition of DNA, the double helix structure of the degeneration, generally high GC content of DNA is not easy to degeneration. The study of base composition provides the basis for further analysis of DNA, preparation of probes, molecular hybridization and other genetic engineering. To this end, we tested the base composition of Paragonimus westermani (3np · w, 2np · w) and Paragonimus yicale (p. I). First, the parasites and inoculation in addition to Yile Village Paragonimus metacercaria collected from Yingkou County, Liaoning Province Shuiyuan Township, the other operations with the literature [Wang Enrong et al: Chinese Journal of Parasitology and Parasitic Diseases 1991; 9 ( 1): 46]. Second, chromosomal DNA preparation According to the method described by Leornard, DNA extraction. The OD260nm / OD280nm ratios were 1.65, 1.73 and 1.75, respectively. The DNA contents ranged from 1.7 to 2.3μg / μl. Content and purity of the experimental requirements.