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采用高效液相色谱荧光检测器(HPLC-FLD)法测定鲈组织中残留的阿维菌素,研究其在鲈体内的富集和消除规律。使用0.5μg/L阿维菌素对初始体重为(120±15)g的鲈连续药浴20 d,停药后,采用高效液相色谱法测定肌肉、肝脏、鳃和血液组织中阿维菌素的残留量。结果表明,阿维菌素能够在鲈肌肉、肝脏、血液和鳃各组织中富集,但不同组织对阿维菌素的富集能力不同,肝脏的蓄积能力最强,而肌肉的蓄积能力最弱。肝脏中阿维菌素最高质量浓度(C max)可达87.36μg/kg,药时曲线下面积(AUC肝脏)为12 977.73(μg/L)·h,而肌肉C max仅为7.74μg/kg,AUC肌肉仅为2 105.75(μg/L)·h,肌肉、鳃、肝脏和血液对阿维菌素的消除半衰期分别为6.9、9.5、8.2和10.7 d。由于影响水生生物药代动力学的因素较多,同种药物在不同水生生物体内的药代动力学参数有很大差异。鲈在15~18℃条件下连续药浴阿维菌素20 d,可食组织肌肉中阿维菌素的残留量40 d后降至检测限以下。本研究为今后在水产养殖中合理使用阿维菌素药物提供了理论依据。
The residues of avermectin in perch tissue were determined by high performance liquid chromatography (HPLC) with fluorescence detector (HPLC-FLD), and the enrichment and elimination of avermectins in perch tissue were studied. The abamectin of 0.5 μg / L was used for 20 days in continuous bath with the initial body weight of (120 ± 15) g. After discontinuation, Abamectin was determined in muscle, liver, gill and blood by high performance liquid chromatography Su residue. The results showed that avermectins can be enriched in muscle, liver, blood and gill tissues, but different tissues have different abilities to avermectin accumulation, the liver has the highest accumulation capacity, and the muscle accumulation capacity is the most weak. The maximal concentration of abamectin in liver was 87.36μg / kg, the area under the curve (AUC liver) was 12 977.73 (μg / L) · h, and the muscle C max was only 7.74μg / kg , The AUC muscle was only 2 105.75 (μg / L) · h, and the elimination half-lives of abamectin in muscles, gills, liver and blood were 6.9, 9.5, 8.2 and 10.7 d, respectively. Due to the many factors affecting the pharmacokinetics of aquatic organisms, the pharmacokinetic parameters of the same drugs in different aquatic organisms vary greatly. The bass was continuously treated with avermectin for 20 d under the conditions of 15-18 ℃. The residues of abamectin in edible tissue muscle decreased below the detection limit after 40 days. This study provides a theoretical basis for the rational use of abamectin in aquaculture in the future.