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本研究采用动式染毒技术,将大鼠分成4个浓度组吸入豆油油烟(油温220℃,观察血中SOD,GSH,MDA含量,肺组织中LDH,ACP,MDA和肺巨噬细胞(PAM)膜脂流动性(LFU)等指标的变化,探讨吸入油烟的急性毒作用机理。实验结果表明:油烟平均浓度为1.05mg/m3时,血浆及肺组织中MDA开始增加(P<0.05),且含量随吸入油烟量增加;平均浓度为9.58mg/m3时,全血SOD活力明显降低(P<0.05)且伴有GSH的下降,同时肺泡灌洗液中PAM膜脂流动性增加(P<0.01)且LDH,ACP酶活力升高(P<0.05)。提示急性吸入豆油油烟时在机体及肺组织中可能产生大量自由基,进一步诱发脂质过氧化反应引起肺组织细胞的破坏,崩解,导致以肺急性炎症为主的机体损伤反应。
In this study, the rats were divided into four concentration groups and inhaled soybean oil fume (temperature 220 ℃). The levels of SOD, GSH, MDA in the blood, LDH, ACP, MDA and pulmonary macrophages (PAM) fluidity (LFU) and so on, and to explore the acute toxicity mechanism of inhalation fumes.The results showed that when the average concentration of fume was 1.05mg / m3, MDA in plasma and lung tissue began to increase (P <0.05 ), And the content increased with the amount of soot inhalation. When the average concentration was 9.58mg / m3, the activity of SOD in whole blood was significantly decreased (P <0.05) and GSH was decreased. At the same time, the flow of PAM membrane lipids (P <0.01), and the activities of LDH and ACP increased (P <0.05), suggesting that a large amount of free radicals may be produced in the body and lung tissues during acute inhalation of soybean oil fume, further inducing lipid peroxidation to cause lung Tissue cell destruction, disintegration, leading to acute lung inflammation-based body damage response.